A novel RAR1 gene, designated MhRAR1, was cloned by the methods of RT-PCR and RACE from Malus hupehensis. The full length sequence of MhRAR1 is 1065 bp with an open reading frame of 678 bp, encoding a protein of 225 amino acids. As found in other plant RAR1 proteins, sequence alignment showed that MhRAR1 protein contains two CHORD domains and one plant-specific CCCH domain. In addition, the MhRAR1 contains conserved strings of invariant cysteine and histidine residues within the CHORD domains and CCCH domain. These results suggested that MhRAR1 protein from M. hupehensis might share the similar function with the Arabidopsis thaliana RAR1 and Hordeum vulgare RAR1, and is an important component of R gene–mediated disease resistance. Phylogenetic analysis revealed that MhRAR1 was closely related to Ricinus communis RAR1. The analysis by qRT-PCR revealed that the expression of MhRAR1 gene was higher in leaves than that in stems and roots. SA, MeJA and ACC treatment induced MhRAR1 expression in stems and roots, but not in leaves. Expression of MhRAR1 was weakly induced in M. hupehensis after infection with Botryosphaeria berengeriana. The cloning and characterization of the MhRAR1 gene will be useful for further studies of biological roles of MhRAR1 in plants.
| Published in | Journal of Plant Sciences (Volume 3, Issue 2) |
| DOI | 10.11648/j.jps.20150302.17 |
| Page(s) | 85-91 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Malus hupehensis, MhRAR1, cDNA Cloning, Expression Pattern
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APA Style
Zhang Ji-Yu, Guo Zhong-Ren. (2015). Molecular Cloning, Characterization and Expression Analysis of MhRAR1 Gene from Malus Hupehensis. Journal of Plant Sciences, 3(2), 85-91. https://doi.org/10.11648/j.jps.20150302.17
ACS Style
Zhang Ji-Yu; Guo Zhong-Ren. Molecular Cloning, Characterization and Expression Analysis of MhRAR1 Gene from Malus Hupehensis. J. Plant Sci. 2015, 3(2), 85-91. doi: 10.11648/j.jps.20150302.17
AMA Style
Zhang Ji-Yu, Guo Zhong-Ren. Molecular Cloning, Characterization and Expression Analysis of MhRAR1 Gene from Malus Hupehensis. J Plant Sci. 2015;3(2):85-91. doi: 10.11648/j.jps.20150302.17
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Download@article{10.11648/j.jps.20150302.17,
author = {Zhang Ji-Yu and Guo Zhong-Ren},
title = {Molecular Cloning, Characterization and Expression Analysis of MhRAR1 Gene from Malus Hupehensis},
journal = {Journal of Plant Sciences},
volume = {3},
number = {2},
pages = {85-91},
doi = {10.11648/j.jps.20150302.17},
url = {https://doi.org/10.11648/j.jps.20150302.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150302.17},
abstract = {A novel RAR1 gene, designated MhRAR1, was cloned by the methods of RT-PCR and RACE from Malus hupehensis. The full length sequence of MhRAR1 is 1065 bp with an open reading frame of 678 bp, encoding a protein of 225 amino acids. As found in other plant RAR1 proteins, sequence alignment showed that MhRAR1 protein contains two CHORD domains and one plant-specific CCCH domain. In addition, the MhRAR1 contains conserved strings of invariant cysteine and histidine residues within the CHORD domains and CCCH domain. These results suggested that MhRAR1 protein from M. hupehensis might share the similar function with the Arabidopsis thaliana RAR1 and Hordeum vulgare RAR1, and is an important component of R gene–mediated disease resistance. Phylogenetic analysis revealed that MhRAR1 was closely related to Ricinus communis RAR1. The analysis by qRT-PCR revealed that the expression of MhRAR1 gene was higher in leaves than that in stems and roots. SA, MeJA and ACC treatment induced MhRAR1 expression in stems and roots, but not in leaves. Expression of MhRAR1 was weakly induced in M. hupehensis after infection with Botryosphaeria berengeriana. The cloning and characterization of the MhRAR1 gene will be useful for further studies of biological roles of MhRAR1 in plants.},
year = {2015}
}
TY - JOUR T1 - Molecular Cloning, Characterization and Expression Analysis of MhRAR1 Gene from Malus Hupehensis AU - Zhang Ji-Yu AU - Guo Zhong-Ren Y1 - 2015/03/26 PY - 2015 N1 - https://doi.org/10.11648/j.jps.20150302.17 DO - 10.11648/j.jps.20150302.17 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 85 EP - 91 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20150302.17 AB - A novel RAR1 gene, designated MhRAR1, was cloned by the methods of RT-PCR and RACE from Malus hupehensis. The full length sequence of MhRAR1 is 1065 bp with an open reading frame of 678 bp, encoding a protein of 225 amino acids. As found in other plant RAR1 proteins, sequence alignment showed that MhRAR1 protein contains two CHORD domains and one plant-specific CCCH domain. In addition, the MhRAR1 contains conserved strings of invariant cysteine and histidine residues within the CHORD domains and CCCH domain. These results suggested that MhRAR1 protein from M. hupehensis might share the similar function with the Arabidopsis thaliana RAR1 and Hordeum vulgare RAR1, and is an important component of R gene–mediated disease resistance. Phylogenetic analysis revealed that MhRAR1 was closely related to Ricinus communis RAR1. The analysis by qRT-PCR revealed that the expression of MhRAR1 gene was higher in leaves than that in stems and roots. SA, MeJA and ACC treatment induced MhRAR1 expression in stems and roots, but not in leaves. Expression of MhRAR1 was weakly induced in M. hupehensis after infection with Botryosphaeria berengeriana. The cloning and characterization of the MhRAR1 gene will be useful for further studies of biological roles of MhRAR1 in plants. VL - 3 IS - 2 ER -
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