Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia

Sena Bayisa; Tadesse Kebede; Gessessew Bugssa; Megbaru Alemu

doi:doi:10.11648/j.sjph.20150301.16

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Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia

Sena Bayisa, Tadesse Kebede, Gessessew Bugssa, Megbaru Alemu

Published in Science Journal of Public Health (Volume 3, Issue 1)

Received: 20 December 2014 Accepted: 4 January 2015 Published: 12 January 2015

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Abstract

Background: The use of RDT (Rapid diagnostic tests) for malaria offers the potential to extend accurate malaria diagnosis to areas where microscopy services are not available. Objective: To evaluate the performance of SD FK80 test kit for the diagnosis of malaria and assess the trend of malaria transmission in Adama Malaria Center, southeast Ethiopia. Methods: A cross sectional study was conducted to evaluate the performance of SD FK80 kit for malaria (P.falciparum/P.vavix) diagnosis November to December 2012 at Adama Malaria Center, Southeastern Oromia. Three hundred eighty four blood samples were collected from febrile patients attending the outpatient department of Adama Malaria Center during the study period. The blood samples were analyzed with microscopy and RDT (SD Bioline P.falciparum /P.vivax) for the detection and identification of Plasmodium parasites. The data was entered into Microsoft® Excel and was transported to SPSS version 17.0 for statistical analysis. Sensitivity, specificity, positive and negative predictive values of the tests was calculated using microscopy as the reference standard. Result: Among the examined individuals, 107(27.9%) were found to be positive for plasmodium infection of which 23.7% were infected with P.vavix, and 3.9% were infected with P.falciparum while 0.3% were mixed infections The sensitivity, specificity, positive predictive value and negative predictive values of the SD Bioline were 90.7%, 96%, 89.8%, and 96.4%, respectively taking blood film as a gold standard. SD Bioline FK80 P.falciparum/P.vivax was performed satisfactorily for the diagnosis of P.falciparum and P.vivax infections. Conclusion: Based on our finding, Rapid test kit is satisfactory for diagnosis of P.falciparum and P.vivax infections. It is a useful adjunct to microscopy especially in our country where there is limited man power and resource.

Published in	Science Journal of Public Health (Volume 3, Issue 1)
DOI	10.11648/j.sjph.20150301.16
Page(s)	31-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Malaria, Microscopy, RDT, P.falciparum, P.vivax

References

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[17]	Biswas S, Tomar D and Rao DN. Investigation of the kinetics of histidine-rich protein 2 and of the antibody responses to this antigen, in a group of malaria patients from India. Ann Trop Med Parasitol. 2005; 99:553-562.
[18]	Chansuda W, Mazie JB, Sinuon M, Awalludin S and Walther HW. A Review of Malaria Diagnostic Tools: Microscopy and Rapid Diagnostic Test (RDT). American J of Tropical Medicine and Hygiene. 2007; 77.
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APA Style

Sena Bayisa, Tadesse Kebede, Gessessew Bugssa, Megbaru Alemu. (2015). Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia. Science Journal of Public Health, 3(1), 31-36. https://doi.org/10.11648/j.sjph.20150301.16

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ACS Style

Sena Bayisa; Tadesse Kebede; Gessessew Bugssa; Megbaru Alemu. Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia. Sci. J. Public Health 2015, 3(1), 31-36. doi: 10.11648/j.sjph.20150301.16

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AMA Style

Sena Bayisa, Tadesse Kebede, Gessessew Bugssa, Megbaru Alemu. Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia. Sci J Public Health. 2015;3(1):31-36. doi: 10.11648/j.sjph.20150301.16

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@article{10.11648/j.sjph.20150301.16,
  author = {Sena Bayisa and Tadesse Kebede and Gessessew Bugssa and Megbaru Alemu},
  title = {Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia},
  journal = {Science Journal of Public Health},
  volume = {3},
  number = {1},
  pages = {31-36},
  doi = {10.11648/j.sjph.20150301.16},
  url = {https://doi.org/10.11648/j.sjph.20150301.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20150301.16},
  abstract = {Background: The use of RDT (Rapid diagnostic tests) for malaria offers the potential to extend accurate malaria diagnosis to areas where microscopy services are not available. Objective: To evaluate the performance of SD FK80 test kit for the diagnosis of malaria and assess the trend of malaria transmission in Adama Malaria Center, southeast Ethiopia. Methods: A cross sectional study was conducted to evaluate the performance of SD FK80 kit for malaria (P.falciparum/P.vavix) diagnosis November to December 2012 at Adama Malaria Center, Southeastern Oromia. Three hundred eighty four blood samples were collected from febrile patients attending the outpatient department of Adama Malaria Center during the study period. The blood samples were analyzed with microscopy and RDT (SD Bioline P.falciparum /P.vivax) for the detection and identification of Plasmodium parasites. The data was entered into Microsoft® Excel and was transported to SPSS version 17.0 for statistical analysis. Sensitivity, specificity, positive and negative predictive values of the tests was calculated using microscopy as the reference standard.  Result: Among the examined individuals, 107(27.9%) were found to be positive for plasmodium infection of which 23.7% were infected with P.vavix, and 3.9% were infected with P.falciparum while 0.3% were mixed infections The sensitivity, specificity, positive predictive value and negative predictive values of the SD Bioline were 90.7%, 96%, 89.8%, and 96.4%, respectively taking blood film as a gold standard. SD Bioline FK80 P.falciparum/P.vivax was performed satisfactorily for the diagnosis of P.falciparum and P.vivax infections. Conclusion: Based on our finding, Rapid test kit is satisfactory for diagnosis of P.falciparum and P.vivax infections. It is a useful adjunct to microscopy especially in our country where there is limited man power and resource.},
 year = {2015}
}

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TY  - JOUR
T1  - Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia
AU  - Sena Bayisa
AU  - Tadesse Kebede
AU  - Gessessew Bugssa
AU  - Megbaru Alemu
Y1  - 2015/01/12
PY  - 2015
N1  - https://doi.org/10.11648/j.sjph.20150301.16
DO  - 10.11648/j.sjph.20150301.16
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 31
EP  - 36
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20150301.16
AB  - Background: The use of RDT (Rapid diagnostic tests) for malaria offers the potential to extend accurate malaria diagnosis to areas where microscopy services are not available. Objective: To evaluate the performance of SD FK80 test kit for the diagnosis of malaria and assess the trend of malaria transmission in Adama Malaria Center, southeast Ethiopia. Methods: A cross sectional study was conducted to evaluate the performance of SD FK80 kit for malaria (P.falciparum/P.vavix) diagnosis November to December 2012 at Adama Malaria Center, Southeastern Oromia. Three hundred eighty four blood samples were collected from febrile patients attending the outpatient department of Adama Malaria Center during the study period. The blood samples were analyzed with microscopy and RDT (SD Bioline P.falciparum /P.vivax) for the detection and identification of Plasmodium parasites. The data was entered into Microsoft® Excel and was transported to SPSS version 17.0 for statistical analysis. Sensitivity, specificity, positive and negative predictive values of the tests was calculated using microscopy as the reference standard.  Result: Among the examined individuals, 107(27.9%) were found to be positive for plasmodium infection of which 23.7% were infected with P.vavix, and 3.9% were infected with P.falciparum while 0.3% were mixed infections The sensitivity, specificity, positive predictive value and negative predictive values of the SD Bioline were 90.7%, 96%, 89.8%, and 96.4%, respectively taking blood film as a gold standard. SD Bioline FK80 P.falciparum/P.vivax was performed satisfactorily for the diagnosis of P.falciparum and P.vivax infections. Conclusion: Based on our finding, Rapid test kit is satisfactory for diagnosis of P.falciparum and P.vivax infections. It is a useful adjunct to microscopy especially in our country where there is limited man power and resource.
VL  - 3
IS  - 1
ER  -

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Author Information

Sena Bayisa

Institute of Biomedical Sciences, College of Health Science, Mekele University, Mekele, Ethiopia
Tadesse Kebede

Department of Microbiology, Immunology and Parasitology (DMIP), School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia
Gessessew Bugssa

Institute of Biomedical Sciences, College of Health Science, Mekele University, Mekele, Ethiopia
Megbaru Alemu

Institute of Biomedical Sciences, College of Health Science, Mekele University, Mekele, Ethiopia

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APA Style

Sena Bayisa, Tadesse Kebede, Gessessew Bugssa, Megbaru Alemu. (2015). Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia. Science Journal of Public Health, 3(1), 31-36. https://doi.org/10.11648/j.sjph.20150301.16

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ACS Style

Sena Bayisa; Tadesse Kebede; Gessessew Bugssa; Megbaru Alemu. Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia. Sci. J. Public Health 2015, 3(1), 31-36. doi: 10.11648/j.sjph.20150301.16

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AMA Style

Sena Bayisa, Tadesse Kebede, Gessessew Bugssa, Megbaru Alemu. Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia. Sci J Public Health. 2015;3(1):31-36. doi: 10.11648/j.sjph.20150301.16

Copy | Download

@article{10.11648/j.sjph.20150301.16,
  author = {Sena Bayisa and Tadesse Kebede and Gessessew Bugssa and Megbaru Alemu},
  title = {Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia},
  journal = {Science Journal of Public Health},
  volume = {3},
  number = {1},
  pages = {31-36},
  doi = {10.11648/j.sjph.20150301.16},
  url = {https://doi.org/10.11648/j.sjph.20150301.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjph.20150301.16},
  abstract = {Background: The use of RDT (Rapid diagnostic tests) for malaria offers the potential to extend accurate malaria diagnosis to areas where microscopy services are not available. Objective: To evaluate the performance of SD FK80 test kit for the diagnosis of malaria and assess the trend of malaria transmission in Adama Malaria Center, southeast Ethiopia. Methods: A cross sectional study was conducted to evaluate the performance of SD FK80 kit for malaria (P.falciparum/P.vavix) diagnosis November to December 2012 at Adama Malaria Center, Southeastern Oromia. Three hundred eighty four blood samples were collected from febrile patients attending the outpatient department of Adama Malaria Center during the study period. The blood samples were analyzed with microscopy and RDT (SD Bioline P.falciparum /P.vivax) for the detection and identification of Plasmodium parasites. The data was entered into Microsoft® Excel and was transported to SPSS version 17.0 for statistical analysis. Sensitivity, specificity, positive and negative predictive values of the tests was calculated using microscopy as the reference standard.  Result: Among the examined individuals, 107(27.9%) were found to be positive for plasmodium infection of which 23.7% were infected with P.vavix, and 3.9% were infected with P.falciparum while 0.3% were mixed infections The sensitivity, specificity, positive predictive value and negative predictive values of the SD Bioline were 90.7%, 96%, 89.8%, and 96.4%, respectively taking blood film as a gold standard. SD Bioline FK80 P.falciparum/P.vivax was performed satisfactorily for the diagnosis of P.falciparum and P.vivax infections. Conclusion: Based on our finding, Rapid test kit is satisfactory for diagnosis of P.falciparum and P.vivax infections. It is a useful adjunct to microscopy especially in our country where there is limited man power and resource.},
 year = {2015}
}

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TY  - JOUR
T1  - Performance of SO Bioline FK80 Test Kit in Diagnosis of Malaria at Adama Malaria Center, Southeast Oromia, Ethiopia
AU  - Sena Bayisa
AU  - Tadesse Kebede
AU  - Gessessew Bugssa
AU  - Megbaru Alemu
Y1  - 2015/01/12
PY  - 2015
N1  - https://doi.org/10.11648/j.sjph.20150301.16
DO  - 10.11648/j.sjph.20150301.16
T2  - Science Journal of Public Health
JF  - Science Journal of Public Health
JO  - Science Journal of Public Health
SP  - 31
EP  - 36
PB  - Science Publishing Group
SN  - 2328-7950
UR  - https://doi.org/10.11648/j.sjph.20150301.16
AB  - Background: The use of RDT (Rapid diagnostic tests) for malaria offers the potential to extend accurate malaria diagnosis to areas where microscopy services are not available. Objective: To evaluate the performance of SD FK80 test kit for the diagnosis of malaria and assess the trend of malaria transmission in Adama Malaria Center, southeast Ethiopia. Methods: A cross sectional study was conducted to evaluate the performance of SD FK80 kit for malaria (P.falciparum/P.vavix) diagnosis November to December 2012 at Adama Malaria Center, Southeastern Oromia. Three hundred eighty four blood samples were collected from febrile patients attending the outpatient department of Adama Malaria Center during the study period. The blood samples were analyzed with microscopy and RDT (SD Bioline P.falciparum /P.vivax) for the detection and identification of Plasmodium parasites. The data was entered into Microsoft® Excel and was transported to SPSS version 17.0 for statistical analysis. Sensitivity, specificity, positive and negative predictive values of the tests was calculated using microscopy as the reference standard.  Result: Among the examined individuals, 107(27.9%) were found to be positive for plasmodium infection of which 23.7% were infected with P.vavix, and 3.9% were infected with P.falciparum while 0.3% were mixed infections The sensitivity, specificity, positive predictive value and negative predictive values of the SD Bioline were 90.7%, 96%, 89.8%, and 96.4%, respectively taking blood film as a gold standard. SD Bioline FK80 P.falciparum/P.vivax was performed satisfactorily for the diagnosis of P.falciparum and P.vivax infections. Conclusion: Based on our finding, Rapid test kit is satisfactory for diagnosis of P.falciparum and P.vivax infections. It is a useful adjunct to microscopy especially in our country where there is limited man power and resource.
VL  - 3
IS  - 1
ER  -

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