Ethanol producing from biomass has become an interesting substitute to petrolium in the struggle against raised levels of fossil carbon dioxide emission to the atmosphere. The biotechnological conversion of biomass into fuels requires hydrolysis of the polysaccharide fraction into monomeric sugars. This study was aimed at the optimization of the processing conditions of the hydrolysis step of bio-ethanol production to produce high fermentable sugar. In this study dilute acid hydrolysis, temprature and residunce time were used as process parameters. Response SurfaceMethodology (RSM) was employed for the optimization of hydrolysis conditions. A 3 (three) level design was used to develop a statistical model for the optimization of process variables. The raw material, P. juliflra stem, was collected from Afar region eastern part of Ethiopia specifically Wrer Agricultural Research Center. The optimal hydrolysis conditions that resulting for the maximum total reducing sugar concentration were at acid concentration; 2% (v/v), temperature; 128.01°C and hydrolysis time; 55 minutes. Under these conditions, the total reducing sugar concentration was obtained to be 184.716mg/g.
| Published in | Science Journal of Energy Engineering (Volume 4, Issue 1) |
| DOI | 10.11648/j.sjee.20160401.11 |
| Page(s) | 1-11 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Ethanol, Prosopis Juliflora, Reducing Sugar, Hydrolysis
| [1] | Admasu, D. (December 2008). the case of Prosopis juliflora in the Afar region of Ethiopia. FARM_Africa for IUCN. |
| [2] | Amarasekara, A. &. (2011). aryl sulfonic acid catalyzed hydrolysis of cellulose in water. Genera applied chemistryl, 417-418, 259-262. |
| [3] | Amartey, S. a. (1996). An improvement in Pichia stipitis fermentation of acidhydrolysed hemicellulose achieved by over-liming (calcium hydroxide treatment) and strain adaptation. Wo. J. Microbiol. Biotechnol. 12, 281-283. |
| [4] | Association of Official Analatical Chemists, o. m. (1972). sample preparation. Washington, D. C. |
| [5] | ASTM D 1105-84. (1993). "Method for Preparation of Extractive-Free Wood.". American Society for Testing and Materials: Annual Book of ASTM standards, Volume 04. 09. Philadelphia, PA. |
| [6] | ASTM. (1995). Standard Test Method for Determination of Acid Insoluble Residues inBiomass. Philadelphia, Pennsylvania: - ASTM E 1721-95. ASTM. |
| [7] | Azhar, A. F. (1981). Factors affecting alcohol fermentation of wood acid hydrolysate. Biotechnol Bioeng. Symp 11, 293-300. |
| [8] | Batista, A., Mustafa, A., McKinnon, J., & Kermasha, S. (2002). In situ ruminal and intestinal nutrient digestibilities of mesquite (Prosopis juliflora) pods. Animal Feed Science and technology, 107-112. |
| [9] | Beck, R., & Clements., D. (1982). Ethanol Production from Cotton Gin Trash. Proceedings of the Symposium on: Cotton Gin Trash Utilization Alternatives. 163-181. |
| [10] | Bienkowski, P. R. (1987). Correlation of glucose (dextrose) degradation at 90 to 190-degrees-C in 0.4 to 20-percent acid. Chem. Eng. Commun. 51 179-192. |
| [11] | Bisaria, V. S. (1981). Biodegradation of cellulosic materials: Substrates, microorganisms, enzymes and products. Enz. Microb. Technol. 3, 90-104. |
| [12] | Brennane, A., Hoagland, W., & Schell, D. (1986). High temperature acid hydrolysis of biomass using an engineering scale plug flow reactor: results of low solids testing. Biotechnology and Bioengineering 17, 53-70. |
| [13] | Brink, D. L. (2007). Alchol production from wood. USA. |
| [14] | Buchert, J. J. (1988). Comparison of Pseudomonas fragi and Gluconobacter oxydans for production of xylonic acid from hemicellulose hydrolysates. Appl. Microbiol. Biotechnol. 28, 367-372. |
| [15] | Burkart, A. (1976). A monograph of the genus Prosopis (Leguminosae subfam. Mimosoideae). Arnold Arbor. 57, 219-249, 450-525. |
| [16] | Canettieri, E. d. (2007). Optimization of acid hydrolysis from the hemicellulosic fraction of Eucalyptus grandis residue using response surface methodology. Bioresource Technology 98, 422-428. |
| [17] | Caraballo, J. (august 2005). Ethanol synthesis. Germany: Birkenfeld Germany. |
| [18] | Chandrakant, P. a. (1998). Simultaneous bioconversion of cellulose and hemicellulose to ethanol. Critical Reviews in Biotechnology 18, 295-331. |
| [19] | Change, C., Cen, P., & Ma, X. (2007). Levulinc acid production from what straw. Bioresource Technology 98, 1448-1453. |
| [20] | Chum, H. e. (1990). Pretreatment-catalyst effects and the combined severity parameter. Applied Biochemistry and Biotechnology 24- 25, 1-14. |
| [21] | Converse et al., A. (1989). Kinetics of thermochemical pretreatment of lignocellulosic materials. Applied Biochemistry and Biotechnology 21, 111-116. |
| [22] | Coppock, D. A. (2005). Agro-Pastoralists Wrath for the Prosopis Tree: the Case of the Il Chamus of Baringo District, Kenya. Utah: ENVS Faculty publication. |
| [23] | Council, W. E. (2007). Survey of Energy Resources. canda: Bioenergy. |
| [24] | Dan, B. (2008). Cellulosic Ethanol. Iowa State Universty. |
| [25] | Deans, J. e. (2003). Comparative growth, biomass production, nutrient use and soil amelioration by nitrogenfixing tree species in semi-arid Senegal. Forest Ecology and Mangement 176, 23-264. |
| [26] | Delgenes, J., Moletta, R., & Navarro, J. (1996). Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis, and Candida shehatae. Enzyme and Microbial Technolgy, pp. 220-225. |
| [27] | Demirbas, A. (2005). Bioethanol from Cellulosic Materials:. A Renewable Motor Fuel from Biomass. Energy Sources 27:., 327-337. |
| [28] | Deshpande, V. V. (1986). Direct conversion of cellulose/ hemicellulose to ethanol by Neurospora crassa. Enz. Microb. Technol. 8, 149. |
| [29] | Dien, B., Ximenes, E., & Bryan, P. (2008). Enzyme characterization for hydrolysis of afex and liquid hot-water pretreated distillers’ grains and their conversion to ethanol. Bioresource Technology 99, 5216-5225. |
| [30] | Dien, B., Cotta, M., & Jeffries, T. (2003). Bacteria engineered for fuel ethanol production: Current status. Applied Microbiology and Biotechnology 63, 258-266. |
| [31] | Duff, S., & Murray, W. (1996). Bioconversion of forest products industry waste cellulosics to fuel ethanol:. A review. Bioresource Technology 55, 1-33. |
| [32] | Durante, T. S. (2011). Economic impact of ethanol production. Maryland: Ethanol Across America. |
| [33] | EEP. (2006). Proceeding of the Workshop on Afar Pastoralist Prosopis Project Immerging Issues. Addis Ababa. |
| [34] | Elander RT, D. B. (2009). Summary of findings from the biomass refining consortium for applied fundamentals and innovation (CAFI): Corn stover pretreatment. 649-659: Cellulose 16. |
| [35] | El-keblawy, A. a.-R. (2006). Germination of invasive P. juliflora (Sw.) DC. Journal of Arid Environment 61:, 555-565. |
| [36] | ESDA, E. s. (2005). Revised Master Schedule for New and Expansion Projects Annual Implementation. Addis Ababa. |
| [37] | Esteghlalian, A. H. (1997). Modeling and optimization of the dilute-sulfuric-acid pretreatment of corn stover, poplar and switchhgrass.. Bioresour. Technol. 59, 129-136. |
| [38] | Felker, P. (1979). Mesquite-An all-purpose leguminous arid-land tree. In: G. A. Ritchie (ed.), New agricultural crops. American Association for the Advancement of Science, 89-132. |
| [39] | Fengel, D., & Wegener, G. (1989.). Wood: Chemistry, Ultrastructure, Reactions. Berlin: de Gruyter. |
| [40] | Galbe, M. a. (2002). A review of the production of ethanol from softwood. Applied Microbiology and Biotechnology 59: 618. |
| [41] | Geirwyr. (1995). S. J., Analytical chemistry of food. Chapman and hall, Glasgow. |
| [42] | Glazer, A., & Nikaido, H. (1995). From biomass to fuel. In Microbial Biotechnology: Fundamentals of Applied Microbiology, 325-391. |
| [43] | Gray, K., & Emptage, M. (2006). Bioethanol. Current opinion in chemistry Biology 10, 141-146. |
| [44] | Himmel, M. e. (2007). Biomass recalcitrance: Engineering plants and enzymes for biofuels production. |
| [45] | Hogsett, D. A. (1992). Direct microbial conversion: Prospects, progress and obstacles. Appl. Biochem. Biotechnol. 34/35, 527-541. |
| [46] | Hopkins, M. (2007). Cooking up a smoky solution. Nature News, doi: 10. 1038/news 070813-1. |
| [47] | Iqbal, K. &. (1983). An analytical study on cell size variation in some arid zone trees of India. Acacia nilotica and Prosopis spicigera. IAWA Bull. n. s. 4, 46-52. |
| [48] | Jackman, E. (1987). Industrial alcohol. In: Basic Biotechnology. London, Academic Press, 39-336. |
| [49] | Jeevan, P., Nelson, R., & Edith Rena, A. (2011). Optimization studies on acid hydrolysis of Corn cob hemicellulosic. Journal of Microbiology and Biotechnology Research 1 (4), 114-123. |
| [50] | Jeffries, T. (2006). Engineering yeasts for xylose metabolism. Current Opinion in Biotechnolgy 17, 320-326. |
| [51] | Kadar, Z. a. (2004). Simultaneous saccharification and fermentation (SSF) of industrial wastes for the production of ethanol. Industrial crops and Products 96, 673-686. |
| [52] | Kailappan, R. G. (2000). Production of activated carbon from prosopis (Prosopis juliflora). Bioresource Technology 75, 241-243. |
| [53] | Kapoor, M. N. (2008). Cost-effective xylanase production from free and immobilized Bacillus pumilus strain mobilized Bacillus strain Mkoo 1 and its application in saccharification prosopis juliflora. Biochem. Eng. J. 38, 88-97. |
| [54] | Kim, J., Lee, Y., & Torget, R. (2001). Cellulose hydrolysis under extremely low sulhuric acid and. Biochem., 331-340. |
| [55] | Klapatch, T. R. (1994). Organism development and characterization for ethanol production using thermophilic bacteria. Appl. Biochem. Biotechnol. 45/46, 209-213. |
| [56] | Klinke, H. B. (2004). Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass. Applied Microbiology and Biotechnology 66, 10-26. |
| [57] | Kootstra, A., Beeftink, H., & Scott, E. (2009). Comparison of dilute mineral and organic acid pretreatment for enzymatic hydrolysis of wheat straw. Biochemical Engineering Journal, Vol. 46, No. 2, 126-131. |
| [58] | Ladisch, M., Mosier, N., & Kim, Y. (2010). Converting cellulose to biofuels. Chemical Engineering Progress 106, 56-63. |
| [59] | Li, W.-Z.-j.-F. C.-q.-C. (2008). Studies of momosaccharide production through lignocellulosic waste hydrolysis using double acid. energy fuels 22 (3), 2015-2021. |
| [60] | Lichit, C. B. (2013). World fuel ethanol analysis and out look. |
| [61] | Licht, F. (2006). World Ethanol Market: The Outlook to 2015. Europe Special Report UK.: Tunbridge Wells, Agra. |
| [62] | Licht, F. (2012). World Ethanol and Biofuels Report. New York: Earth Policy Institute with data: W. W. Norton & Company. |
| [63] | Lin, Y. a. (2006). Ethanol fermentation from biomass resources: current state and prospects. Applied Microbiology and Biotechnology 69, 627-642. |
| [64] | Luo, C. e. (2002). Identification of potential fermentation inhibitors in conversion ofhybrid poplar hydrolyzate to ethanol. Biomass and Bioenergy 22, 125-138. |
| [65] | Lynd, L., Weimer, P., van Zyl, W., & Pretorius, I. (2002). Microbial cellulose utilization: Fundamentals and biotechnology. Microbiology and Molecular Biology Reviews 66, 506-577. |
| [66] | Mabee, W. E. (2007). Policy Options to Support Biofuel Production. Biofuels, 329-357. |
| [67] | Matura, U., Nuanphan, N., & Woatthichai, N. (2012). Reducing Sugar Production from Durian Peel by Hydrochloric Acid Hydrolysis. World Academy of Science, Engineering and Technology 69, 444-449. |
| [68] | McKendry, P.. (2002). Energy production from biomass (part 1): overview of biomass. Bioresource Technology 83, 37-46. |
| [69] | Melsert, R. M. (2007). Energy Optimization of the Production of Cellulosic Ethanol from Southern Pine. Thesis for the Degree Masters of Science in the School of Mechanical Engineering. Georgia Institute of Technology, Georgia., 78. |
| [70] | Mitiku, S. G. (2008). THE ECOLOGICAL DISTRIBUTION AND SOCIO-ECONOMIC IMPACTS OF Prosopis juliflora (Sw.) DC. IN THE AMIBARA WOREDA, AFAR NATIONAL REGION STATE. Addis Ababa: Addis Ababa University thesis. |
| [71] | Moiser, N., Wyman, C., Dale, B., Elander, R., Lee, Y., Holtzapple, M., et al. (2005). Features of promising technologies for pretreatment oflignocellulosic biomass. Bioresource technolgy 96, 673-686. |
| [72] | MoME. (2007). (Ministry of Mines and Energy) Biofuel Development and Utlization strategy. Addis Ababa. |
| [73] | Negusu. (2009). Potential biofuel production from prosopis julifora. Addis Ababa: Addis Ababa Universty. |
| [74] | Nigam, J. N. (2001). Ethanol production from wheat straw hemicellulose hdrolysate by Pichia Stipitis. J. Biotechnol. 87 (1) 17-21. |
| [75] | Nigus, W. (2011). Ethanol production from Bamboo. Addis Ababa: Addis Ababa University. |
| [76] | Nishikawa, N. K. (1988). The influence of lignin degradation products on xylose fermentation by Klebsiella pneumoniae. Appl. Microbiol. Biotechnol. 27, 549-552. |
| [77] | Ogunkunle, T. O. (2005). Production of Glucose from Lignocellulosic under Extremely Low Acid. Journal of Applied Sciences 5 (1), 15-17. |
| [78] | Ojumu, T., & Ogunkunle, O. (2005). Production of Glucose from Lignocellulosic under Extremely Low Acid. Journal of Applied Sciences 5 (1), 15-17. |
| [79] | Olsson, L., & Hahn-Hägerdal., B. (1996). Fermentation of lignocellulosic hydrolysates forethanol production. Enzyme and Microbial Technology 18, 312-331. |
| [80] | Onuki, S. (2005). Bioethanol, Industrial production process and recent studies. USA. |
| [81] | Otero, J., Panagiotou, G., & Olsson, L. (2007). Fueling Industrial Biotechnology Growth with Bioethanol. Adv Biochem Eng Biotechnol 108, 1-40. |
| [82] | Patel, R. R., & Safaya, V. (1986). The Role of Prosopis in Wasteland Development. Jivrajbhai Patel Agroforestry Center. |
| [83] | Periyasamy, S. V. (2009). Production of Bioethanol from Sugar Molasses Using S. cerevisiae. Modern Appl. Science 3:, 32-36. |
| [84] | Philippidis, G. (1993). Study of the Enzymatic-Hydrolysis of Cellulose for Production of Fuel Ethanol by the Simultaneous Saccharification and Fermentation Process. Biotechnology and Bioengineering. Biotechnology and Bioengineering 41, 846-85. |
| [85] | prasad, S., Singh, A., & Joshi, H. (2007). Ethanol as an alternative fuel from agricultural, industrial and urban residues. Resources Conservation and Recycling 50, 1-39. |
| [86] | Puneet, D., Janaki, R., Alavalapati, & Pankaj, L. (2009). Cellulosic Ethanol Production. Florida: School of Forest Resources and Conservation, University of Florida. |
| [87] | Rajput, S. &. (1986). Some studies on the utilisation of bio-mass of Prosopis spp. India: In: V. J. Patel (ed.), The role of Prosopis in wasteland development. Javrajbhai Patel AgroforestryCenter. Surendrabag, Gujarat. In Pasiecznik et al. (2001). |
| [88] | Renewable fuels association. (2012). Ethano industry outlook. USA. |
| [89] | Riveros, F. (1992). The genus Prosopis and its potential to improve vestock production in arid and semi arid regions. FAO Animal Production and Health paper, 257-276. |
| [90] | Saeman, J. F. (1945). Kinetics of wood saccharification - Hydrolysis of cellulose and decomposition of sugars in dilute acid at high temperature. Ind. Eng. Chem. 37, 43-52. |
| [91] | Saucedo, l. J., Castro, M. A., Rico, J., & campos-Garcia, J. (2010). Optimization of Acid hydrolysis of bagasse from Agave tequilana Weber. Revista Mexicana de ingenieria Quimica, vol. 9, num. 1, 95. |
| [92] | Sedlak, M., NWY, H., Ladisch, M., Mosier, N., & Case, E. (2011). Biofuels from Cellulosic Feedstocks. Elsevier, 52-61. |
| [93] | Shene, C. B. (2001). Zymomonas mobilis CP 4 fed-batch fermentations of glucose-fructose mixtures to ethanol and sorbitol. Applied Microbiology and biotechnology 57, 323-328. |
| [94] | Shetie, G. (2008). The Ecological Distribution and Socio-Economic impacts of P. juliflora (Sw.) DC. in the Amibara Woreda, Afar National Regional State. Addis Ababa: Master Thesis. AAU. |
| [95] | Shiferaw, H. (2004). Some biological characteristics that foster the invasion of P. juliflora (Sw.) DC. At Middle Awash Rift Valley, North-eastern Ethiopia. Journal of Arid Environment 58, 34-153. |
| [96] | Sirmah, P., Muisu, F., Mburu, F., & Dumarcay, S. (2008). Evaluation of Prosopis juliflora Properties as Alternative to Wood Shortage in Kenya. Bois Et Forets Des Tropiques 298 (4), 25-35. |
| [97] | Sivers, M. V., & Zacchi, G. (1996). Ethanol from lignocellulosics: a review of economy. Bioresource Technology, 131-140. |
| [98] | Solomon, B. D. (2007). Grain and cellulosic ethanol: History, economics, and energy policy. 416-425. Biomass & Bioenergy 31. |
| [99] | Sun, Y. a. (2002). Hydrolysis of lignocellulosic materials for ethanolproduction. Bioresource Technology 83, 1-11. |
| [100] | Szczodrak, J. a. (1996.). Technology for conversion of lignocellulosic biomass to ethanol. Biomass and Bioenergy 10:, 367-375. |
| [101] | Taherzadeh, M. J. (1997). Grain and cellulosic ethanol. Industrial and Engineering Chemistry Reasearch 36, 4659-4665. |
| [102] | Taherzadeh, M. J. (2000). Physiological effects of 5-hydroxymethylfurfural on Saccharomyces cerevisiae," l. Appl. Microbio Biotechnol. 53 701-708. |
| [103] | Talebnia, F. (2008). Ethanol production from cellulosic biomassby Encapsulated saccharomyces cervisisae. Sweden: Chalmers University of Technology. |
| [104] | Tano, M., & Buzato. (2003). Effect of the presence of initialethanol production in sugar cane juice fermented by Zymomonas mobilis. Brazilian Journal of microbiology 34, 242-244. |
| [105] | TEFERA, N. (2009). potential production of biofuel from prosopis julifora pod. Addis Ababa Universty: Addis Ababa Universty. |
| [106] | Tessema, T. (2007). The Prospects of Biological Controls of Weeds in Ethiopia. Ethiopian Journal of weed Management 1, 63-67. |
| [107] | Tewodros Wakie, P. E. (july 2012). Utilization Assessment of Prosopis juliflora in Afar Region, Ethiopia. Colorado: Colorado State University, thesis. |
| [108] | Tilahun, S. L., & A. A. (2012). Modeling the Expansion of Prosopis Juliflora and Determining its Optimum Utilization Rate to Control itsInvasion in Afar Regional State of Ethiopia. International Journal of Applied Mathematical Research, 726-743. |
| [109] | Torget, R., Himmel, M., & Grohmann, K. (1991). Dilute sulfuric acid pretreatment of hardwood bark. Bioresource Technol. 35, 239-246. |
| [110] | Vish Noi, N. (1996). Advanced prctical organic chemistry. Viskas publisher house PVT limited. |
| [111] | Werpy, T., & Petersen, G. (2004). Top value added chemicals from biomass volume I: Results of screening for potential candidates from sugars and synthesis gas. Available at: www1.eere.energy.gov/biomass/pdfs/35523.pdf. |
| [112] | Zhu, J. p. (2010). Woody biomass pretreatment for cellulosic ethanol production: technology and energy consumption evaluation. Bioresource Technology 101, 992-5002. |
APA Style
Biniyam Tefera Amdebrhan, Sisay Asfaw, Gizachew Assefa. (2016). Acid Hydrolysis Optimization of Prosopis Juliflora Stem for Bioethanol Production. Science Journal of Energy Engineering, 4(1), 1-11. https://doi.org/10.11648/j.sjee.20160401.11
ACS Style
Biniyam Tefera Amdebrhan; Sisay Asfaw; Gizachew Assefa. Acid Hydrolysis Optimization of Prosopis Juliflora Stem for Bioethanol Production. Sci. J. Energy Eng. 2016, 4(1), 1-11. doi: 10.11648/j.sjee.20160401.11
AMA Style
Biniyam Tefera Amdebrhan, Sisay Asfaw, Gizachew Assefa. Acid Hydrolysis Optimization of Prosopis Juliflora Stem for Bioethanol Production. Sci J Energy Eng. 2016;4(1):1-11. doi: 10.11648/j.sjee.20160401.11
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.sjee.20160401.11,
author = {Biniyam Tefera Amdebrhan and Sisay Asfaw and Gizachew Assefa},
title = {Acid Hydrolysis Optimization of Prosopis Juliflora Stem for Bioethanol Production},
journal = {Science Journal of Energy Engineering},
volume = {4},
number = {1},
pages = {1-11},
doi = {10.11648/j.sjee.20160401.11},
url = {https://doi.org/10.11648/j.sjee.20160401.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjee.20160401.11},
abstract = {Ethanol producing from biomass has become an interesting substitute to petrolium in the struggle against raised levels of fossil carbon dioxide emission to the atmosphere. The biotechnological conversion of biomass into fuels requires hydrolysis of the polysaccharide fraction into monomeric sugars. This study was aimed at the optimization of the processing conditions of the hydrolysis step of bio-ethanol production to produce high fermentable sugar. In this study dilute acid hydrolysis, temprature and residunce time were used as process parameters. Response SurfaceMethodology (RSM) was employed for the optimization of hydrolysis conditions. A 3 (three) level design was used to develop a statistical model for the optimization of process variables. The raw material, P. juliflra stem, was collected from Afar region eastern part of Ethiopia specifically Wrer Agricultural Research Center. The optimal hydrolysis conditions that resulting for the maximum total reducing sugar concentration were at acid concentration; 2% (v/v), temperature; 128.01°C and hydrolysis time; 55 minutes. Under these conditions, the total reducing sugar concentration was obtained to be 184.716mg/g.},
year = {2016}
}
TY - JOUR T1 - Acid Hydrolysis Optimization of Prosopis Juliflora Stem for Bioethanol Production AU - Biniyam Tefera Amdebrhan AU - Sisay Asfaw AU - Gizachew Assefa Y1 - 2016/08/06 PY - 2016 N1 - https://doi.org/10.11648/j.sjee.20160401.11 DO - 10.11648/j.sjee.20160401.11 T2 - Science Journal of Energy Engineering JF - Science Journal of Energy Engineering JO - Science Journal of Energy Engineering SP - 1 EP - 11 PB - Science Publishing Group SN - 2376-8126 UR - https://doi.org/10.11648/j.sjee.20160401.11 AB - Ethanol producing from biomass has become an interesting substitute to petrolium in the struggle against raised levels of fossil carbon dioxide emission to the atmosphere. The biotechnological conversion of biomass into fuels requires hydrolysis of the polysaccharide fraction into monomeric sugars. This study was aimed at the optimization of the processing conditions of the hydrolysis step of bio-ethanol production to produce high fermentable sugar. In this study dilute acid hydrolysis, temprature and residunce time were used as process parameters. Response SurfaceMethodology (RSM) was employed for the optimization of hydrolysis conditions. A 3 (three) level design was used to develop a statistical model for the optimization of process variables. The raw material, P. juliflra stem, was collected from Afar region eastern part of Ethiopia specifically Wrer Agricultural Research Center. The optimal hydrolysis conditions that resulting for the maximum total reducing sugar concentration were at acid concentration; 2% (v/v), temperature; 128.01°C and hydrolysis time; 55 minutes. Under these conditions, the total reducing sugar concentration was obtained to be 184.716mg/g. VL - 4 IS - 1 ER -
Email: