Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species

Tenderwealth Clement Jackson; Timma Otobong Uwah; Nonye Linda Ifekpolugo; Nsikak Anthony Emmanuel

doi:doi:10.11648/j.nano.20180602.12

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Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species

Tenderwealth Clement Jackson, Timma Otobong Uwah, Nonye Linda Ifekpolugo, Nsikak Anthony Emmanuel

Published in American Journal of Nano Research and Applications (Volume 6, Issue 2)

Received: 18 May 2018 Accepted: 14 June 2018 Published: 7 July 2018

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Abstract

Synthesis of nanoparticles was done by green method. Silver nitrate was used as the silver precursor, while the fruit juices of the citrus fruits (Citrus sinensis, Citrus limetta, Citrus aurantifolia, Citrus paradisi) were used as reducing and stabilizing agents. The nanoparticle formation was confirmed with the visible colour change from colourless to characteristic reddish brown. the surface plasmon resonance peak was at 451 and 452 nm for the silver nanoparticles The antimicrobial activities of these nanoparticles were studied against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis. Generally, MIC values of the samples against the microorganism tested ranged from 25-100mg/ml. Pseudomonas aeruginosa was the most sensitive (while Staphylococcus aureus and Bacillus subtilis were the least sensitive to the silver nanoparticles).

Published in	American Journal of Nano Research and Applications (Volume 6, Issue 2)
DOI	10.11648/j.nano.20180602.12
Page(s)	54-59
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Silver Nanoparticles, Green Method, Characterization, Antimicrobial

References

[1]	Roy S, Das T. K., (2015). Plant mediated green synthesis of silver nanoparticles. A Review. Int. J. Plant Biol. Res. 3(3):1044-1055.
[2]	Sathishkumar M, Sneha K, Won S. W., Cho C. W., Kim S, Yun Y. S. (2009). Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf. B. Biointerfaces 73(2):332-338.
[3]	Sachin S, Anupama P, Meenal K (2012). Biosynthesis of silver nanoparticles by marine bacterium, Idiomarine Sp. PR-58-5. Bull. Mater. Sci. 35 (7): 1201-1205.
[4]	Rao B, Tang R. C., (2017). Green synthesis of silver nanoparticles using aqueous Eriobotyra japonica leaf extract. Adv. Nat. Sci. Nanosci. Nanotechnol. 8(2017) 015014 (8p).
[5]	Kumar P, Suranjit P, Darshit P, Patel P, Selvaraj K, Prasad S (2011). Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antibacterial effect. Afr. J. Biotechnol. 10(41):8122-8130.
[6]	Xin Y, Qingbiao L. I., Huixuan W, Jiale H, Liqin L, Wenta W, Daohua S, Yuanbo S, James B, Luwei H, Yuanpeg W, Ning H, Lishan J (2010). Green synthesis of palladium nanoparticles using broth of Cinnamomum camphora leaf. J. Nanopart. Res. 12: 1589-1598.
[7]	Jackson T. C., Agboke A, Jackson I, Ekpuk E (2016). Biosynthesis of silver nanoparticles using Murraya koenigii and Acacia Gum. Int. J. Nanomed. 8: 1507-1516.
[8]	Prabhu N., Raj T., Gowri D., Yamuna K,. Ayisha S., Joseph S., and Puspha D (2010) Synthesis of silver phyto nanoparticles and their antibacterial efficacy. Dig. J. Nanomat. Bios; 5(1); 185.
[9]	Vivekanandhan S, Misra M, Mohanty AK (2010) Novel Glycine Max (soyabean) leaf extract based biological process for the functionalization of carbon nanotubes with silver nanoparticles. Nanosci. Nanotechnol. let 2(3): 240.
[10]	Hugund BS, Dhulappanavar GR, Ayachit NH (2015) Comparative Evaluation of Antibacterial Activity of Silver Nanoparticles Biosynthesized using Fruit Juices. J Nanomed Nanotechnol 2015, 6:271. doi:10.4172/2157-7439.1000271
[11]	CLSI, Performance Standards for Antimicrobial Disk Susceptibility Tests, Approved Standard, 7th Edition; CLSI document MO2-A11. Clinical and Laboratory Standards Institute, 950 West Valley Road, Suite 2500, Wayne, Pennsylvania 19087, USA, 2012.
[12]	Vijayakumar, M., et al. (2013) Biosynthesis, Characterisation and Anti-bacterial Effect of Plant-Mediated Silver Nanoparticlles Using Artemisia nilagirica. Industrial Crops and Products, 41, 235-240.
[13]	Alzahrani, E. and Welham, K. (2014) Optimization Preparation of the Biosynthesis of Silver Nanoparticles Using Watermelon and Study of Its Antibacterial Activity. International Journal of Basic and Applied Sciences, 3, 392-400.
[14]	Alzahrani, E. (2015) Ecofriendly Production of Silver Nanoparticles from Peel of Tangerine for Degradation of Dye. World Journal of Nano Science and Engineering, 5, 10-16.
[15]	Sudha, M., Murugan, B. (2015) Orange Seed Mediated Green Synthesis Silver Nanoparticles. International Journal of Biosciences and Nanosciences. Volume 2 (11), 2015, pp. 215-220.

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APA Style

Tenderwealth Clement Jackson, Timma Otobong Uwah, Nonye Linda Ifekpolugo, Nsikak Anthony Emmanuel. (2018). Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species. American Journal of Nano Research and Applications, 6(2), 54-59. https://doi.org/10.11648/j.nano.20180602.12

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ACS Style

Tenderwealth Clement Jackson; Timma Otobong Uwah; Nonye Linda Ifekpolugo; Nsikak Anthony Emmanuel. Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species. Am. J. Nano Res. Appl. 2018, 6(2), 54-59. doi: 10.11648/j.nano.20180602.12

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AMA Style

Tenderwealth Clement Jackson, Timma Otobong Uwah, Nonye Linda Ifekpolugo, Nsikak Anthony Emmanuel. Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species. Am J Nano Res Appl. 2018;6(2):54-59. doi: 10.11648/j.nano.20180602.12

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@article{10.11648/j.nano.20180602.12,
  author = {Tenderwealth Clement Jackson and Timma Otobong Uwah and Nonye Linda Ifekpolugo and Nsikak Anthony Emmanuel},
  title = {Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species},
  journal = {American Journal of Nano Research and Applications},
  volume = {6},
  number = {2},
  pages = {54-59},
  doi = {10.11648/j.nano.20180602.12},
  url = {https://doi.org/10.11648/j.nano.20180602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.20180602.12},
  abstract = {Synthesis of nanoparticles was done by green method. Silver nitrate was used as the silver precursor, while the fruit juices of the citrus fruits (Citrus sinensis, Citrus limetta, Citrus aurantifolia, Citrus paradisi) were used as reducing and stabilizing agents. The nanoparticle formation was confirmed with the visible colour change from colourless to characteristic reddish brown. the surface plasmon resonance peak was at 451 and 452 nm for the silver nanoparticles The antimicrobial activities of these nanoparticles were studied against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis. Generally, MIC values of the samples against the microorganism tested ranged from 25-100mg/ml. Pseudomonas aeruginosa was the most sensitive (while Staphylococcus aureus and Bacillus subtilis were the least sensitive to the silver nanoparticles).},
 year = {2018}
}

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TY  - JOUR
T1  - Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species
AU  - Tenderwealth Clement Jackson
AU  - Timma Otobong Uwah
AU  - Nonye Linda Ifekpolugo
AU  - Nsikak Anthony Emmanuel
Y1  - 2018/07/07
PY  - 2018
N1  - https://doi.org/10.11648/j.nano.20180602.12
DO  - 10.11648/j.nano.20180602.12
T2  - American Journal of Nano Research and Applications
JF  - American Journal of Nano Research and Applications
JO  - American Journal of Nano Research and Applications
SP  - 54
EP  - 59
PB  - Science Publishing Group
SN  - 2575-3738
UR  - https://doi.org/10.11648/j.nano.20180602.12
AB  - Synthesis of nanoparticles was done by green method. Silver nitrate was used as the silver precursor, while the fruit juices of the citrus fruits (Citrus sinensis, Citrus limetta, Citrus aurantifolia, Citrus paradisi) were used as reducing and stabilizing agents. The nanoparticle formation was confirmed with the visible colour change from colourless to characteristic reddish brown. the surface plasmon resonance peak was at 451 and 452 nm for the silver nanoparticles The antimicrobial activities of these nanoparticles were studied against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis. Generally, MIC values of the samples against the microorganism tested ranged from 25-100mg/ml. Pseudomonas aeruginosa was the most sensitive (while Staphylococcus aureus and Bacillus subtilis were the least sensitive to the silver nanoparticles).
VL  - 6
IS  - 2
ER  -

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Author Information

Tenderwealth Clement Jackson

Department of Pharmaceutics and Pharmaceutical Technology, University of Uyo, Uyo, Nigeria
Timma Otobong Uwah

Department of Pharmaceutics and Pharmaceutical Technology, University of Uyo, Uyo, Nigeria
Nonye Linda Ifekpolugo

Department of Pharmaceutics and Pharmaceutical Technology, University of Uyo, Uyo, Nigeria
Nsikak Anthony Emmanuel

Department of Pharmaceutics and Pharmaceutical Technology, University of Uyo, Uyo, Nigeria

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Plain Text BibTeX RIS

APA Style

Tenderwealth Clement Jackson, Timma Otobong Uwah, Nonye Linda Ifekpolugo, Nsikak Anthony Emmanuel. (2018). Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species. American Journal of Nano Research and Applications, 6(2), 54-59. https://doi.org/10.11648/j.nano.20180602.12

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ACS Style

Tenderwealth Clement Jackson; Timma Otobong Uwah; Nonye Linda Ifekpolugo; Nsikak Anthony Emmanuel. Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species. Am. J. Nano Res. Appl. 2018, 6(2), 54-59. doi: 10.11648/j.nano.20180602.12

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AMA Style

Tenderwealth Clement Jackson, Timma Otobong Uwah, Nonye Linda Ifekpolugo, Nsikak Anthony Emmanuel. Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species. Am J Nano Res Appl. 2018;6(2):54-59. doi: 10.11648/j.nano.20180602.12

Copy | Download

@article{10.11648/j.nano.20180602.12,
  author = {Tenderwealth Clement Jackson and Timma Otobong Uwah and Nonye Linda Ifekpolugo and Nsikak Anthony Emmanuel},
  title = {Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species},
  journal = {American Journal of Nano Research and Applications},
  volume = {6},
  number = {2},
  pages = {54-59},
  doi = {10.11648/j.nano.20180602.12},
  url = {https://doi.org/10.11648/j.nano.20180602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.20180602.12},
  abstract = {Synthesis of nanoparticles was done by green method. Silver nitrate was used as the silver precursor, while the fruit juices of the citrus fruits (Citrus sinensis, Citrus limetta, Citrus aurantifolia, Citrus paradisi) were used as reducing and stabilizing agents. The nanoparticle formation was confirmed with the visible colour change from colourless to characteristic reddish brown. the surface plasmon resonance peak was at 451 and 452 nm for the silver nanoparticles The antimicrobial activities of these nanoparticles were studied against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis. Generally, MIC values of the samples against the microorganism tested ranged from 25-100mg/ml. Pseudomonas aeruginosa was the most sensitive (while Staphylococcus aureus and Bacillus subtilis were the least sensitive to the silver nanoparticles).},
 year = {2018}
}

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TY  - JOUR
T1  - Comparison of Antimicrobial Activities of Silver Nanoparticles Biosynthesized from Some Citrus Species
AU  - Tenderwealth Clement Jackson
AU  - Timma Otobong Uwah
AU  - Nonye Linda Ifekpolugo
AU  - Nsikak Anthony Emmanuel
Y1  - 2018/07/07
PY  - 2018
N1  - https://doi.org/10.11648/j.nano.20180602.12
DO  - 10.11648/j.nano.20180602.12
T2  - American Journal of Nano Research and Applications
JF  - American Journal of Nano Research and Applications
JO  - American Journal of Nano Research and Applications
SP  - 54
EP  - 59
PB  - Science Publishing Group
SN  - 2575-3738
UR  - https://doi.org/10.11648/j.nano.20180602.12
AB  - Synthesis of nanoparticles was done by green method. Silver nitrate was used as the silver precursor, while the fruit juices of the citrus fruits (Citrus sinensis, Citrus limetta, Citrus aurantifolia, Citrus paradisi) were used as reducing and stabilizing agents. The nanoparticle formation was confirmed with the visible colour change from colourless to characteristic reddish brown. the surface plasmon resonance peak was at 451 and 452 nm for the silver nanoparticles The antimicrobial activities of these nanoparticles were studied against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus subtilis. Generally, MIC values of the samples against the microorganism tested ranged from 25-100mg/ml. Pseudomonas aeruginosa was the most sensitive (while Staphylococcus aureus and Bacillus subtilis were the least sensitive to the silver nanoparticles).
VL  - 6
IS  - 2
ER  -

Copy | Download